P12.11.B The role of miRNAs in glioma in response to hypoxia

Abstract Background Hypoxia, low oxygen, is a microenvironment that promotes tumour progression, particularly in gliomas. The regulation of many biological processes are maintained by miRNAs. hypoxia status glioma cells effects the affecting expression individual miRNAs.The aims this study identifying significant miRNAs either under or overexpressed compared to normoxia and further exploring effect on miR-92a-3p miR-149-5p gliomas apoptotic cellular senescence pathways. Material Methods A range were used for screening including primary cell lines: GIN28 GIN31; low-grade LGG19 LGG24; paediatric line: SF188 commercially available glioblastoma line U87. These culture at both 1% (hypoxia) 20% (normoxia) oxygen levels. Screening was achieved quantitative polymerase chain reaction (qPCR) using miRNA specific primers. Knockdowns/in transfecting with mimics inhibitors. Caspase-glo assay assess apoptosis. Results 92a-3p found be downregulated upregulated respectively normoxia. particular also have multiple targets apoptosis senescence. Conclusion 90 among different categories highlighted Using lines identified hypoxia-affected glioblastomas. This discovery will increase our knowledge understanding which may aid direct targeted therapy conquer